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Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...

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Related Experiment Video

Updated: Dec 1, 2025

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Capturing Cardiogenesis in Gastruloids.

Giuliana Rossi¹, Nicolas Broguiere¹, Matthew Miyamoto²

¹Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Vaud, Switzerland.

|November 11, 2020

Summary

This summary is machine-generated.

Mouse embryonic stem cells form axially patterned organoids that mimic early heart development. These gastruloids generate cardiovascular progenitors and beating cardiac tissue, offering a new model for organogenesis research.

Keywords:

3D cardiac tissue cardiac organoid cardiogenesis development embryonic organoids heart in vitro organogenesis

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Last Updated: Dec 1, 2025

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Area of Science:

Developmental Biology
Stem Cell Biology
Regenerative Medicine

Background:

Organoids are valuable models for studying development, physiology, and disease.
Current organoid systems often lack the complex tissue-tissue interactions crucial for native organogenesis.

Purpose of the Study:

To develop a novel organoid model that recapitulates key aspects of early heart organogenesis.
To investigate the morphogenetic potential of mouse embryonic stem cells in a self-organizing system.

Main Methods:

Utilizing axially patterned embryonic organoids (gastruloids) derived from mouse embryonic stem cells (mESCs).
Observing the spatiotemporal development and self-organization of cardiovascular progenitors within the gastruloids.

Main Results:

mESCs in gastruloids successfully underwent fundamental steps of early heart organogenesis with in-vivo-like fidelity.
Generated cardiovascular progenitors, including first and second heart fields, which self-organized into a cardiac crescent-like structure.
Formed a beating cardiac tissue adjacent to a primitive gut-like tube, separated by an endocardial-like layer.

Conclusions:

Mouse embryonic stem cell-derived gastruloids demonstrate significant potential for recapitulating complex organogenesis.
This platform enables the study of coordinated multi-tissue development and heart formation with high spatiotemporal accuracy.
The developed model offers a powerful tool for detailed and high-throughput investigation of heart development and related diseases.