Resorufin-based responsive probes for fluorescence and colorimetric analysis.

Area of Science:

• Analytical Chemistry
• Biochemistry
• Chemical Biology

Background:

• Fluorescence imaging offers high selectivity and sensitivity for real-time biological molecule detection.
• Resorufin is a versatile fluorophore with high quantum yield and suitability for responsive probe design.

Purpose of the Study:

• To review the advancements in resorufin-based fluorescent probes.
• To summarize their applications in detecting diverse analytes.
• To discuss future perspectives in this field.

Main Methods:

• Literature review of resorufin-based fluorescent probes.
• Analysis of probe chemical structures and response mechanisms.
• Evaluation of detection limits and practical applications.

Main Results:

• Resorufin probes are effective for detecting cations, anions, sulfur species, small molecules, and macromolecules.
• Various chemical structures and response mechanisms have been developed.
• Demonstrated utility in bioassays and in situ imaging.

Conclusions:

• Resorufin-based probes show significant potential for biological detection.
• Further development is needed to address current challenges.
• These probes are crucial for advancing bioimaging and diagnostics.