A Combination MAI and MALDI Vacuum Source Operational from Atmospheric Pressure for Fast, Robust, and Sensitive...

Area of Science:

• Analytical Chemistry
• Mass Spectrometry

Background:

• Previous vacuum matrix-assisted ionization (vMAI) achieved low attomole detection with no carryover.
• Probe sample introduction devices were used for matrix/analyte introduction into mass spectrometer vacuum.

Purpose of the Study:

• To develop a novel vacuum ionization source for mass spectrometry.
• To achieve the sensitivity and robustness of probe devices with faster multisample introduction and sequential ionization.
• To enable both vMAI and vacuum matrix-assisted laser desorption/ionization (vMALDI) with rapid switching capabilities.

Main Methods:

• A new vacuum ionization source was designed and implemented.
• The source supports both vMAI and vMALDI ionization modes.
• Samples are introduced into the mass spectrometer vacuum for spontaneous or laser-assisted ionization.

Main Results:

• The new source duplicates the sensitivity and robustness of previous probe devices.
• Fast sequential ionization was achieved, with sample acquisition in as little as 1 second per sample.
• Switching between vMAI and vMALDI modes takes only a few seconds.

Conclusions:

• The novel vacuum ionization source enhances molecular analyses by combining vMAI and vMALDI.
• Rapid sequential acquisition and quick plate exchange facilitate high-throughput mass spectrometry.
• The developed prototype suggests potential for low-cost, high-throughput automated systems.