Hands on Native Mass Spectrometry Analysis of Multi-protein Complexes
Stéphane Erb1, Sarah Cianférani2, Julien Marcoux3
1Laboratoire de Spectroméérie de Masse BioOrganique, Universitéde Strasbourg, CNRS, IPHC UMR7178, 67000 Strasbourg, France.
Abstract:
By maintaining intact multi-protein complexes in the gas-phase, native mass spectrometry provides their molecular weight with very good accuracy compared to other methods (typically native PAGE or SEC-MALS) (Marcoux and Robinson, Structure 21:1541-1550, 2013). Besides, heterogeneous samples, in terms of both oligomeric states and ligand-bound species can be fully characterized. Here we thoroughly describe the analysis of several oligomeric protein complexes ranging from a 16 = kDa dimer to a 801-kDa tetradecameric complex on different instrumental setups.
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