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Coated electrospun polyamide-6/chitosan scaffold with hydroxyapatite for bone tissue engineering.
Xiaolian Niu1, Miao Qin1, Mengjie Xu1
1Research Center for Nano-Biomaterials and Regenerative Medicine, Department of Biomedical Engineering, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, People's Republic of China.
Biomedical Materials (Bristol, England)
|December 28, 2020
Summary
This study developed mineralized Polyamide-6/Chitosan (PA6/CS) scaffolds using electrospinning. These biocompatible scaffolds mimic natural bone extracellular matrix (ECM) and promote cell growth for bone tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Chemistry
Background:
- Polyamide-6 (PA6) offers stability in body fluid, similar to collagen.
- Chitosan (CS) provides suitable biodegradation for bone regeneration, mimicking extracellular matrix (ECM) polysaccharides.
- Electrospun fibers offer nanoscale structure, high porosity, and large surface area, simulating natural ECM.
Purpose of the Study:
- To fabricate electrospun PA6/CS scaffolds for bone tissue engineering.
- To enhance mechanical properties and biocompatibility.
- To mineralize scaffolds using simulated body fluid (SBF) for hydroxyapatite (HA) layer formation.
Main Methods:
- Electrospinning technology to fabricate PA6/CS fibrous scaffolds.
- Immersion in 1.5× simulated body fluid (1.5SBF) for mineralization and HA layer growth.
- Characterization of scaffold nanostructure, mechanical properties, and mineral content.
- Cell culture studies with MC3T3-E1 cells to assess biocompatibility.
Main Results:
- Electrospun PA6/CS scaffolds (60-260 nm diameter) mimic ECM nanostructure.
- 10PA6/CS scaffolds exhibited tensile strength of 12.67 ± 2.31 MPa and modulus of 95.52 ± 6.78 MPa.
- Mineralized scaffolds showed uniform HA distribution (approx. 40% mineral content) in a honeycomb structure.
- Mineralized scaffolds were non-cytotoxic and promoted MC3T3-E1 cell attachment and proliferation.
Conclusions:
- Fabricated mineralized PA6/CS scaffolds meet mechanical and biocompatibility requirements for bone tissue engineering.
- The mild mineralization process allows HA layer formation without surface modification.
- The composite scaffolds demonstrate excellent potential for promoting bone regeneration.
