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A Pseudoalteromonas Clade with Remarkable Biosynthetic Potential
Rocky Chau1, Leanne A Pearson2, Jesse Cain2
1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Kensington, NSW, Australia.
Applied and Environmental Microbiology
|January 5, 2021
Summary
Pseudoalteromonas bacteria, found in blue-ringed octopuses, produce diverse bioactive compounds. Genomic analysis revealed novel pathways for natural products, highlighting their untapped potential.
Area of Science:
- Marine microbiology
- Natural product chemistry
- Genomics
Background:
- Pseudoalteromonas species are known producers of bioactive compounds via nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs).
- Strain HM-SA03 was isolated from the blue-ringed octopus (Hapalochlaena sp.).
Purpose of the Study:
- To perform biochemical and genomic analysis of Pseudoalteromonas sp. strain HM-SA03.
- To identify and characterize secondary metabolite biosynthesis gene clusters.
- To investigate the distribution of these gene clusters across the Pseudoalteromonas genus.
Main Methods:
- Genome mining for NRPS/PKS gene clusters.
- Biochemical analysis of alterochromide production using liquid chromatography-mass spectrometry.
- Phylogenetic analysis of Pseudoalteromonas strains.
- Comparative analysis of gene cluster density across genomes.
Main Results:
- Seven putative NRPS/PKS gene clusters were identified in HM-SA03, including those for alterochromides, pseudoalterobactins, alteramides, and four novel compounds.
- A novel siderophore biosynthesis gene cluster with an unprecedented NRPS-PKS-NRPS-PKS-NRPS-PKS-NRPS architecture was discovered.
- Alterochromide production was confirmed in HM-SA03.
- Phylogenetic analysis revealed a clade of Pseudoalteromonas strains with significantly higher biosynthetic gene cluster density (average 10 clusters/genome) compared to others (average 3 clusters/genome).
Conclusions:
- Pseudoalteromonas sp. strain HM-SA03 is a metabolically rich organism with potential for novel natural product discovery.
- The identified gene clusters, including a unique siderophore pathway, expand our understanding of Pseudoalteromonas secondary metabolism.
- A specific phylogenetic clade within Pseudoalteromonas exhibits exceptional biosynthetic capabilities, underscoring the genus's potential.
- These findings highlight Pseudoalteromonas as a promising source for novel natural products.
Keywords:
AlteromonasPseudoalteromonasantiSMASHbacteriocingenomelanthipeptidenonribosomal peptidepolyketidesiderophoreMore Related Videos
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