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Updated: Nov 22, 2025

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Miniaturized modular-array fluorescence microscopy.

Jeonghwan Son¹, Biagio Mandracchia¹, Shu Jia¹

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.

Biomedical Optics Express

|January 7, 2021

Summary

This summary is machine-generated.

We developed miniaturized modular-array fluorescence microscopy (MAM) for advanced live-cell imaging. This compact system enables flexible, high-resolution, and parallelized in situ cellular analysis, overcoming limitations of current technologies.

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Last Updated: Nov 22, 2025

Lensless Fluorescent Microscopy on a Chip

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Published on: August 17, 2011

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

Biomedical Engineering
Cell Biology
Microscopy Technology

Background:

Live-cell imaging is crucial for studying cellular behaviors.
Portable imaging platforms offer adaptability and accessibility but face limitations in workflow compatibility and parallelization.
Existing methods struggle with up-right physiological imaging and simultaneous data acquisition.

Purpose of the Study:

To introduce a miniaturized modular-array fluorescence microscopy (MAM) system for compact and flexible live-cell imaging.
To overcome limitations in conventional live-cell imaging, including workflow compatibility and parallel data acquisition.
To provide a cost-effective and accessible solution for in situ single-cell imaging and analysis.

Main Methods:

Developed an up-right modular microscopy architecture using gradient-index (GRIN) objectives.
Integrated individually-addressed illumination and acquisition components within each module.
Enabled parallel data acquisition using an array of modular devices in conventional cell chambers.

Main Results:

Achieved high fluorescence sensitivity and efficiency.
Demonstrated exquisite spatiotemporal resolution (∼3 µm, up to 60 Hz).
Confirmed compatibility with conventional cell culture assays and physiological imaging.

Conclusions:

The miniaturized modular-array fluorescence microscopy (MAM) system offers a promising solution for time-lapse in situ single-cell imaging.
The system's parallelization and flexibility enhance data acquisition capabilities.
MAM advances portable microscopy for broader applications in cell-based assays and biological research.