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Three-Dimensional Microfibrous Bundle Structure Fabricated Using an Electric Field-Assisted/Cell Printing Process for
1Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, South Korea.
ACS Biomaterials Science & Engineering
|January 9, 2021
Summary
This study developed a 3D fibrous scaffold using electrohydrodynamics and cell printing. The aligned structure and collagen bioink effectively promoted skeletal muscle tissue regeneration in vitro.
Area of Science:
- Tissue Engineering
- Biomaterials Science
- Regenerative Medicine
Background:
- Biomimetic scaffolds are crucial for creating cellular microenvironments in tissue engineering.
- Anisotropic topographical cues are vital for effective muscle tissue regeneration.
Purpose of the Study:
- To fabricate a 3D fibrous bundle structure using electrohydrodynamics and cell printing.
- To investigate the potential of this structure for skeletal muscle tissue regeneration.
Main Methods:
- Fabrication of a 3D fibrous bundle structure via electrohydrodynamics.
- Utilizing myoblast-laden collagen bioink for cell printing.
- Uniaxial stretching to achieve aligned topographical cues.
- Optimizing myoblast release from bioink for cell attachment.
Main Results:
- The 3D fibrous bundle structure promoted cell proliferation and myotube formation in vitro.
- Aligned topographical cues and collagen biocompatibility synergistically enhanced myotube development.
- Increased expression of myogenic genes (Myf5, Myh2, MyoD, Myogenin) confirmed myogenesis.
Conclusions:
- The developed 3D fibrous bundle structure is a feasible platform for skeletal muscle tissue regeneration.
- The combination of aligned cues and biocompatible materials enhances muscle tissue formation.
- This approach holds promise for advancing skeletal muscle regenerative therapies.
