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Cryo-focussed ion beam in Life Sciences (and beyond)

D A M de Winter1, C D J Parmenter2

  • 1Faculty of Geosciences, Department of Hydrogeology, Utrecht University, Utrecht, The Netherlands.

Journal of Microscopy
|January 23, 2021
PubMed
Summary

No abstract available in PubMed .

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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