Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Yeast biomimetic iron‑titanium oxides for electrochemical biosensing.

Biophysical chemistry·2026

Same author

Enantioselective transplacental transfer of p-phenylenediamine quinones.

Environmental pollution (Barking, Essex : 1987)·2026

Same author

A Guanine-Enhanced Graphene-DNA Paper-Based Sensing Platform Enabling Sensitive Hg<sup>2+</sup> Detection.

Biosensors·2026

Same author

Corrigendum to 'Research progress of single molecule sensors based on DNA platform' [Talanta 302 (2026) 129449].

Talanta·2026

Same author

Cyclic lipopeptides from <i>Bacillus amyloliquefaciens</i> D-1 control leaf spot in <i>Pseudostellaria heterophylla</i> via enhancing host immunity and inhibiting <i>Alternaria alternata</i>.

Frontiers in microbiology·2026

Same author

Highly Sensitive Detection of Chymotrypsin Using Gold Nanoclusters with Peptide Sensors.

Micromachines·2026

Same journal

The Comparative Study of Two New 6-Hydrazinylpurine Derivatives as "Turn-On" Fluorescence Chemosensors for the Detection of Zn<sup>2</sup>.

Luminescence : the journal of biological and chemical luminescence·2026

Same journal

Investigation of Zirconium-Lithium-Barium Oxides (ZrO<sub>2</sub>.Li<sub>2</sub>O.BaO) Nanocomposites' Optical, Fluorescence, and Thermophysical Characteristics for Optoelectronics and LEDs.

Luminescence : the journal of biological and chemical luminescence·2026

Same journal

Eco-Friendly Spectrofluorimetric Determination of Apixaban Using SDS-Enhanced Fluorescence in Pharmaceutical Forms and Plasma.

Luminescence : the journal of biological and chemical luminescence·2026

Same journal

Spectrofluorimetric Determination of Silodosin Using NBD-Cl Reagent: Applications to Capsules and Content Uniformity Testing.

Luminescence : the journal of biological and chemical luminescence·2026

Same journal

Low-Cost Lanthanum Nickel Manganese Oxide-Based Photodetector With PCBM and CuI Transport Layers for Eco-Friendly Applications.

Luminescence : the journal of biological and chemical luminescence·2026

Same journal

Sensitive Fluorescence Detection Method for Dinotefuran Using Carbon Quantum Dots as Probe Derived From Glucose and Diethylenetriamine.

Luminescence : the journal of biological and chemical luminescence·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 19, 2025

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Published on: December 2, 2022

Protein detection based on rolling circle amplification sensors.

Haixia Shi¹, Jingjie Cui², Husseini Sulemana³

¹P. E. Department of Jiangsu University, Zhenjiang, China.

Luminescence : the Journal of Biological and Chemical Luminescence

|January 27, 2021

Summary

This summary is machine-generated.

Rolling circle amplification (RCA) generates large DNA templates for sensitive protein detection using optical and electrochemical sensors. This review covers RCA sensor advances, mechanisms, and future outlooks.

Keywords:

RCA protein detection sensors

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Related Experiment Videos

Last Updated: Nov 19, 2025

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Published on: December 2, 2022

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Area of Science:

Biotechnology
Analytical Chemistry
Molecular Biology

Background:

Rolling circle amplification (RCA) is a DNA synthesis process.
RCA generates large DNA templates for signal amplification.
RCA is an isothermal process driven by DNA polymerases.

Purpose of the Study:

To review advances in RCA-based sensors for protein detection.
To discuss the advantages and detection mechanisms of RCA sensors.
To outline challenges and future perspectives in RCA sensor development.

Main Methods:

Review of existing literature on RCA-based sensors.
Analysis of signal amplification strategies (optical, electrochemical).
Discussion of detection mechanisms and sensor performance.

Main Results:

RCA enables sensitive protein detection through signal amplification.
Various optical and electrochemical sensors based on RCA have been developed.
RCA offers advantages for sensitive biomolecule detection.

Conclusions:

RCA-based sensors show significant promise for sensitive protein detection.
Understanding RCA mechanisms is key to developing improved sensors.
Future research should address current challenges for broader application.