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Assembly and Characterization of Polyelectrolyte Complex Micelles
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P-selectin targeting polysaccharide-based nanogels for miRNA delivery
Fernanda C Moraes1, Laura Marcelo Forero Ramirez1, Rachida Aid1
1Université de Paris, LVTS, INSERM U1148, Université Sorbonne Paris Nord, F-75018 Paris, France.
International Journal of Pharmaceutics
|February 4, 2021
Summary
Researchers developed novel nanogels using natural polymers and genipin crosslinking. These biocompatible nanogels effectively deliver miRNA for potential atherothrombotic disease therapeutics.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery
Background:
- Atherothrombotic diseases pose significant health challenges, necessitating innovative therapeutic strategies.
- MicroRNA (miRNA) therapeutics offer a promising avenue for treating complex diseases.
- Developing biocompatible and efficient delivery systems for miRNAs is crucial.
Purpose of the Study:
- To synthesize and characterize novel nanogels for miRNA delivery.
- To evaluate the biocompatibility and platelet-binding capacity of the nanogels.
- To explore the potential of these nanogels as a platform for treating atherothrombotic diseases.
Main Methods:
- Preparation of nanogels via polyelectrolyte complexation of aminated pullulan and fucoidan, followed by genipin crosslinking.
- Characterization of nanogel size, zeta potential, and surface charge under varying pH conditions.
- In vitro evaluation of nanogel binding to activated human platelets and cytocompatibility with human endothelial cells.
- Assessment of miRNA loading and intracellular delivery efficiency using fluorescence microscopy.
Main Results:
- Homogeneously distributed genipin crosslinked nanogels (G-PECs) with a mean hydrodynamic diameter of ~155 nm were successfully prepared in aqueous media.
- G-PECs exhibited excellent cytocompatibility with human endothelial cells and low hemolysis rates, indicating good biocompatibility.
- Surface charge modification under acidic conditions enabled efficient miRNA incorporation via electrostatic interactions.
- Fluorescence microscopy confirmed the ability of G-PECs to deliver labeled miRNA into cells.
Conclusions:
- The developed G-PECs are biocompatible nanosystems suitable for miRNA encapsulation and delivery.
- These nanogels show potential as a targeted therapeutic platform for atherothrombotic diseases by targeting P-selectin.
- Further investigation into the biological activity of delivered miRNA is warranted to fully realize their therapeutic potential.
