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Wei Wei1, Yan-Zhen Ye2, Xiao-Yu Zhou2

  • 1Cord Blood Stem Cell Bank of Guangdong Province, Guagnzhou 510663, Guangdong Province, China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|February 8, 2021
PubMed
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Anticoagulant volume impacts umbilical cord blood (UCB) quality. Higher UCB volumes correlate with increased total nucleated cells, viability, CFU-GM, and CD34+ cells, meeting clinical standards even in smaller volumes.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Transfusion Medicine

Background:

  • Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells.
  • The anticoagulant used during UCB collection can influence its quality and cellular yield.
  • Optimizing anticoagulant protocols is crucial for maximizing the therapeutic potential of UCB units.

Purpose of the Study:

  • To investigate the effect of anticoagulant volume on the quality of collected umbilical cord blood (UCB).
  • To analyze key quality parameters including cell counts, viability, and stem cell markers.
  • To determine optimal collection parameters for clinical UCB banking.

Main Methods:

  • 6060 cord blood units (CBUs) were categorized into five groups based on anticoagulant volume ratios.

Related Experiment Videos

  • Evaluated pre-cryopreservation total nucleated cell (pre-TNC) count, nucleated cell viability (VNC), CFU-GM, and CD34+ cell percentages.
  • Statistical analysis was performed to assess correlations between anticoagulant volume and UCB quality metrics.

    • Main Results:

    • Pre-TNC, VNC, CFU-GM, and CD34+ percentages showed a positive correlation with increasing UCB volume (r values ranging from 0.9024 to 0.9937).
    • The minimum UCB volume group demonstrated TNC of (2.57±0.89)×10^8, VNC over 95%, CFU-GM of 89/×10^5, and CD34+ of (0.30±0.19)%.
    • All evaluated quality parameters remained within acceptable ranges for clinical administration, even in the smallest volume groups.

    Conclusions:

    • Within a certain anticoagulant volume, decreasing UCB volume leads to a reduction in pre-TNC, VNC, CFU-GM, and CD34+ percentages.
    • Despite the decrease, UCB units collected with smaller volumes still meet clinical administration requirements.
    • These findings support the clinical usability of UCB units across a range of collected volumes when appropriate anticoagulant protocols are used.