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Microarray Data Mining and Preliminary Bioinformatics Analysis of Hepatitis D Virus-Associated Hepatocellular
Zhe Yu1, Xuemei Ma2, Wei Zhang2
1Peking University 302 Clinical Medical School, Beijing 100039, China.
Biomed Research International
|February 10, 2021
Summary
Chronic hepatitis delta virus (HDV) infection worsens hepatitis B virus (HBV) infection and increases hepatocellular carcinoma (HCC) risk. This study identified seven key genes, including CDCA8, involved in HDV-associated HCC oncogenesis and patient survival.
Area of Science:
- Hepatology and Oncology
- Molecular Biology
- Genomics
Background:
- Chronic hepatitis delta virus (HDV) infection exacerbates hepatitis B virus (HBV) infection.
- HDV infection is linked to an increased risk of hepatocellular carcinoma (HCC).
- Limited data exists on HDV's specific role in HCC oncogenesis.
Purpose of the Study:
- To investigate the molecular mechanisms of HDV-associated hepatocarcinogenesis.
- To identify key genes and pathways involved in HDV-related HCC pathogenesis.
- To screen for novel molecular targets in HDV-associated HCC.
Main Methods:
- Analysis of three microarray datasets (GSE55092, GSE98383) and TCGA RNA-sequencing data.
- Identification of 948 differentially expressed genes (DEGs) in HDV-associated HCC.
- Gene Ontology (GO) and KEGG pathway enrichment analysis, WGCNA, and PPI network construction.
Main Results:
- Seven key genes (3 known, 4 novel) associated with nucleoplasm, cell cycle, DNA replication, and mitosis were identified.
- CDCA8 and HCC stage were independent prognostic factors for overall survival in HDV-associated HCC.
- Hub genes from PPI and WGCNA modules were validated using an independent HCC dataset.
Conclusions:
- The identified genes provide insights into HDV's role in HCC carcinogenesis.
- CDCA8 is a potential prognostic biomarker for HDV-associated HCC.
- Understanding these mechanisms can aid in developing targeted therapies for HDV-related liver cancer.
