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Updated: Nov 17, 2025

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

High-Throughput Synthesis of Liposome Using an Injection-Molded Plastic Micro-Fluidic Device.

Sang-Won Woo^1,2, Yun Kyong Jo³, Yeong-Eun Yoo¹

¹Korea Institute of Machinery and Materials, 156 Gajeongbuk-ro, Yusung-Gu, Daejeon 34103, Korea.

|February 12, 2021

Summary

This study introduces a novel plastic micro-channel device for mass-producing liposomes. The device utilizes micro-nozzle arrays for efficient mixing and synthesis, yielding liposomes of approximately 230-260 nm.

Keywords:

injection molding liposome micro-channel micro-nozzle array plastic

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Last Updated: Nov 17, 2025

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

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Published on: March 3, 2020

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

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Published on: March 17, 2023

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

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Published on: May 16, 2022

Area of Science:

Biotechnology
Materials Science
Chemical Engineering

Background:

Liposome synthesis is crucial for drug delivery and other applications.
Existing methods for liposome production can be inefficient for large-scale manufacturing.

Purpose of the Study:

To design and fabricate a cost-effective plastic micro-channel device for mass production of liposomes.
To optimize liposome synthesis using microfluidic technology.

Main Methods:

A plastic micro-channel device was designed with T-junction micro-nozzle arrays (5 μm) and micro-channels (100 μm depth).
The device featured multiple micro-channel units (approx. 2400 micro-nozzles each) for mixing solutions and collecting liposome suspension.
Mass production of the plastic device was achieved via injection molding, with the stamper fabricated using UV lithography and nickel electroforming.
Microfluidic characteristics were validated using colored water, and L-α-phosphatidylcholine (SOY PC) liposomes were synthesized.

Main Results:

The fabricated device demonstrated effective flow and mixing of solutions within the micro-channels.
Soybean phosphatidylcholine (SOY PC) liposomes were successfully synthesized.
Liposome size ranged from approximately 230 to 260 nm, influenced by the lipid solution's flow rate.

Conclusions:

The developed plastic micro-channel device offers a scalable and efficient platform for liposome mass production.
Microfluidic technology provides precise control over liposome synthesis, enabling tunable particle size.