Genomics Integrated Systems Transgenesis (GENISYST) for gain-of-function disease modelling in Göttingen Minipigs

Area of Science:

• Biotechnology
• Animal Models
• Genetics

Background:

• Göttingen Minipigs share human-like traits, making them ideal for translational research and disease modeling.
• Transgenic minipig models are crucial for replicating human genetic diseases.
• Current methods for generating transgenic minipigs are time-consuming (2-3 years).

Purpose of the Study:

• To develop a rapid and efficient technology platform for generating transgenic Göttingen Minipigs.
• To overcome the lengthy development times associated with current transgenic animal generation.
• To expand the utility of minipigs in disease modeling and drug discovery.

Main Methods:

• Development of the GENISYST (Genomics Integrated Systems Transgenesis) technology platform.
• Utilized advanced genetic techniques for precise animal modifications.
• Proof-of-concept generation of transgenic minipigs expressing green fluorescent protein (GFP).

Main Results:

• Successful generation of transgenic Göttingen Minipigs using the GENISYST platform.
• Demonstrated efficient, one-step generation of minipigs with tailored genetic modifications.
• Confirmed GFP expression in multiple disease-relevant tissues, including liver, heart, kidney, lungs, and CNS.

Conclusions:

• The GENISYST platform offers a feasible and efficient solution for rapid transgenic minipig generation.
• This technology significantly reduces the time and effort required for creating disease models.
• GENISYST is a valuable tool for advancing human disease modeling, drug discovery, and xenotransplantation research.