ENZYMATIC ACTIVITY IN MICROSOMES, LIPID PEROXIDATION OF MICE HEPATOCYTES UNDER THE SODIUM FLUORIDE

Fluorine is one of the most widespread and necessary microelements for the body of animals and humans, which is necessary in a clearly limited amount. Different concentrations of fluorine can affect the state of lipid peroxidation, as well as the functional state of the microsomes of liver hepatocytes. The studies were carried out on mature Wistar rats weighing 180-220 g. Animals were inoculated with an aqueous solution of sodium fluoride once a day for 60 days at doses of 1/10, 1/100 and 1/1000 DL50, which was 20 mg/kg, 2 mg/kg and 0,2 mg/kg body weight. Control rats received drinking water. Each group consisted of 10 animals, the studies of indicators were carried out on days 10, 20, 30, 50 and 60. The induction of free radical processes by sodium fluoride was confirmed using a chemiluminescent reaction of blood serum, the amount of diene conjugates in rat liver tissue homogenates was assessed spectrophotometrically, the content of TBA reactants in rat liver tissue homogenates was determined by the reaction of malondialdehyde and thiobarbituric acid (TBA). The level of chiff bases was determined with a spectrofluorometer, subcellular fractions of the liver were isolated by the method of differentiated centrifugation. An increase in the level of the indicator at a dose of 1/10 and 1/100 DL50 of the intensity of lemiluminescence on the 30th day and its decrease on the 60th day was established.Increase in the activity of NAD (P) H-cytochrome c reductase in the microsomal fraction of the liver at the beginning of the study and a gradual decrease on the 50th and 60th days when using both dosages. The same dynamics was observed for NADH-cytochrome c reductase. With respect to diene conjugates of TBA-reactants and chiff bases, a tendency to increase was observed at all periods of the experiment. Indicators of cytochrome P-450 cytochrome b5 were increased up to 30 days and gradually decreased by 60 days. Long-term administration of sodium fluoride can cause the formation of toxic products and a decrease in the activity of enzymes of the microsomal membrane of hepatocytes.