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Demonstration of negative tissue charges by means of polyethyleneimine-metal complexes
U Pfüller1, K Ichev, W Ovtscharoff
1Staatliches Institut für Immunpräparate und Nährmedien, Berlin, German Democratic Republic.
Histochemistry
|January 1, 1988
Abstract:
Three polyethyleneimine-metal complexes were synthesized and cytochemically tested for demonstration of negatively charged sites. For this purpose rat cerebral cortex synaptosomes were used. It was established that both types of polyethyleneimine-copper complexes labeled the synaptosomal membrane and synaptic vesicles with electron-dense granules, whereas the polyethyleneimine-lead complex marked the anionic sites with amorphous electron-dense material.