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A magnetic relaxation switch sensor for determination of 17β-estradiol in milk and eggs based on
Xin Wang1, Kaili Pei1, Hanying Sun1,2
1School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.
Background:
A simple and rapid detection method for 17β-estradiol (E2 ) in complex food matrix is greatly desirable. A magnetic relaxation switch (MRS) sensor for detecting E2 based on the aptamer-functionalized gold-coated iron oxide (Fe3 O4 @Au) nanocomposite was designed in this study. Fe3 O4 @Au nanoparticles (NPs) played as a 'switch' between dispersed and aggregated states, while aptamer served as the recognition unit.
Results:
According to the sensing effect of monocomponent relaxation time (T2W ) for E2 , the volume ratio of aptamers to Fe3 O4 @Au, the sodium chloride (NaCl) concentration, the concentration of Fe3 O4 @Au@Apt, and reaction time were optimized to be 4:1, 0.03 mol L-1 , 4 μmol L-1 and 15 min, respectively. For the analysis of food sample, the E2 was quantified over a concentration range of 1 to 100 nmol L-1 with a detection limit of 7.6 nmol L-1 for milk samples, while a linearity range of 20 to 100 nmol L-1 and a detection limit of 8.57 nmol L-1 for egg samples.
Conclusion:
These results exhibited that the MRS sensor could be a promising platform for the rapid detecting of E2 in food sample. © 2021 Society of Chemical Industry.
