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Immuno-gold Techniques in Biomedical Sciences

Minkyo Jung1, Tae Keun Kim2, Chan-Gi Pack2,3

  • 1Neural Circuit Research Group, Korea Brain Research Institute, Daegu, South Korea.

Advances in Experimental Medicine and Biology
|April 9, 2021
PubMed

Since their development in the 1960s, immuno-gold techniques have been steadily used in biomedical science, because these techniques are applicable to all kinds of antigens, from viruses to animal tissues. Immuno-gold staining exploits antigen-antibody reactions and is used to investigate locations and interactions of components in the ultrastructure of tissues, cells, and particles. These methods are increasingly used with advanced technologies, such as correlative light and electron microscopy and cryo-techniques. In this protocol, we introduce the principles and technical details of recent advances in this area and discuss their advantages and limitations.

Keywords:
Chemical fixationCryo-fixationImmuno-goldPost-embeddingPre-embedding

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