Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Generation of Functional Oligodendrocyte Progenitor Cells Through Serial Replating of iPSC-Derived NPC Spheres.

Cells·2026

Same author

Visualizing Biomolecules within Living Microglia in Complex Environments Using a Clickable Small Fluorogenic Compound.

ACS chemical neuroscience·2026

Same author

Metformin attenuates cuprizone-induced mitochondrial dysfunction and senescence-associated changes in primary neuronal cells.

Cell structure and function·2026

Same author

Evaluating the Effect of Sorafenib on Gd-EOB-DTPA-mediated Contrast Enhancement: An Experimental Study using DCE-MRI.

Molecular imaging and biology·2026

Same author

Improved conductive carbon nanotube tape using natural cellulose fibrils for array tomography.

Applied microscopy·2026

Same author

3-Dimensional reconstruction reveals frequent intraluminal growth of submucosal veins in surgically resected pT1 colorectal cancers.

Journal of pathology and translational medicine·2026

Same journal

Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

Advances in experimental medicine and biology·2026

Same journal

Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

Advances in experimental medicine and biology·2026

Same journal

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026

Same journal

Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

Advances in experimental medicine and biology·2026

Same journal

PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

Advances in experimental medicine and biology·2026

Same journal

Dynamic Assemblies in Genome Maintenance.

Advances in experimental medicine and biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 9, 2025

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Immuno-gold Techniques in Biomedical Sciences.

Minkyo Jung¹, Tae Keun Kim², Chan-Gi Pack^2,3

¹Neural Circuit Research Group, Korea Brain Research Institute, Daegu, South Korea.

Advances in Experimental Medicine and Biology

|April 9, 2021

Summary

This summary is machine-generated.

Immuno-gold techniques utilize antigen-antibody reactions for ultrastructural analysis in biomedical science. This protocol details recent advances, applications, and limitations of these versatile immuno-staining methods.

Keywords:

Chemical fixation Cryo-fixation Immuno-gold Post-embedding Pre-embedding

More Related Videos

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Published on: February 18, 2016

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Published on: April 3, 2013

Related Experiment Videos

Last Updated: Nov 9, 2025

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Published on: February 18, 2016

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Published on: April 3, 2013

Area of Science:

Biomedical Science
Cell Biology
Microscopy

Background:

Immuno-gold techniques have been a staple in biomedical science since the 1960s.
These methods are broadly applicable to diverse biological samples, including viruses and animal tissues.
They are crucial for investigating the ultrastructure of cells, tissues, and particles.

Purpose of the Study:

To introduce the principles and technical details of recent advances in immuno-gold staining.
To discuss the advantages and limitations of these updated immuno-gold techniques.
To provide a protocol for researchers utilizing advanced microscopy and cryo-techniques.

Main Methods:

Exploitation of antigen-antibody reactions for specific labeling.
Application in conjunction with advanced technologies like correlative light and electron microscopy.
Integration with cryo-techniques for enhanced ultrastructural preservation.

Main Results:

Demonstration of the versatility and broad applicability of immuno-gold staining.
Highlighting the synergy of immuno-gold techniques with modern imaging modalities.
Discussion of the benefits and drawbacks of recent methodological improvements.

Conclusions:

Immuno-gold staining remains a powerful tool for ultrastructural investigation.
Recent advancements enhance the capabilities and applications of these techniques.
Understanding the principles and technical details is key to successful implementation.