Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.5K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rack1 mediates tyrosine phosphorylation of Anxa2 by Src and promotes invasion and metastasis in drug-resistant breast cancer cells.

Breast cancer research : BCR·2019
Same author

Rack1 mediates Src binding to drug transporter P-glycoprotein and modulates its activity through regulating Caveolin-1 phosphorylation in breast cancer cells.

Cell death & disease·2019
Same author

Dynamic Parameter Identification for a Manipulator with Joint Torque Sensors Based on an Improved Experimental Design.

Sensors (Basel, Switzerland)·2019
Same author

Juglone eliminates MDSCs accumulation and enhances antitumor immunity.

International immunopharmacology·2019
Same author

Visceral adipogenesis inhibited by Pref-1 is associated with peritoneal angiogenesis.

Nephrology (Carlton, Vic.)·2019
Same author

Spatial distribution of parabens, triclocarban, triclosan, bisphenols, and tetrabromobisphenol A and its alternatives in municipal sewage sludges in China.

The Science of the total environment·2019
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Nov 9, 2025

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

17.9K

Robust and efficient single-pixel image classification with nonlinear optics.

Santosh Kumar, Ting Bu, He Zhang

    Optics Letters
    |April 15, 2021
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a hybrid image classifier for rapid high-resolution image analysis. It significantly enhances classification accuracy and robustness, even for noisy or occluded images.

    More Related Videos

    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
    07:13

    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

    Published on: May 16, 2022

    2.1K
    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
    09:19

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

    Published on: August 29, 2025

    312

    Related Experiment Videos

    Last Updated: Nov 9, 2025

    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    17.9K
    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
    07:13

    Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

    Published on: May 16, 2022

    2.1K
    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
    09:19

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

    Published on: August 29, 2025

    312

    Area of Science:

    • Computer Vision
    • Signal Processing
    • Machine Learning

    Background:

    • High-resolution image processing demands efficient and accurate classification methods.
    • Existing techniques may struggle with noisy or occluded data, limiting their practical applications.

    Purpose of the Study:

    • To develop a hybrid image classifier for fast and robust processing of high-resolution images.
    • To improve classification accuracy and performance in challenging imaging conditions.

    Main Methods:

    • A hybrid approach combining feature-sensitive image upconversion, single-pixel photodetection, and deep learning.
    • Utilizing partial Fourier transform for extracting signature features in both spatial and Fourier domains.
    • Testing on Modified National Institute of Standards and Technology (NIST) handwritten digit datasets.

    Main Results:

    • Achieved a classification accuracy of 99.23%, a significant improvement from 81.25% with Fourier-domain processing alone.
    • Demonstrated 83% accuracy for highly contaminated images with a signal-to-noise ratio as low as -17 dB.
    • Validated performance through simulation and testing on benchmark datasets.

    Conclusions:

    • The proposed hybrid classifier offers superior accuracy and robustness for high-resolution image recognition.
    • The method shows potential for applications in lidar data processing, occluded target identification, and atmospheric monitoring.
    • This approach addresses limitations of current methods in handling degraded image quality.