3D-printed fluidic electrochemical microcell for sequential injection/stripping analysis of heavy metals

In this paper, a 3D-printed microfluidic device is described which is suitable for sequential injection/anodic stripping voltammetric (SIA-ASV) determination of Pb(II) and Cd(II). The fluidic device is manufactured by 3D-printing in a single-step using a dual extruder 3D printer. The device is composed of a microfluidic cell (printed from a non-conductive polylactic acid (PLA) filament) and of 3 electrodes (printed from a conductive carbon-loaded PLA filament) which are integrated within the fluidic cell. During the preconcentration step, a zone of the sample (containing the target cations) and a zone of Bi(III) solution are mixed on-line and the cations are reduced on the working electrode forming a bismuth alloy. The detection step involves a voltametric scan in static solution, in which the accumulated metals are oxidized while the oxidation current is monitored. After the optimization of the relevant parameters, the limit of detection is 0.38 μg L^-1 for Pb(II) and 0.57 μg L^-1 for Cd(II), while the within-device repeatability and the between-device reproducibility are lower than 4.5% (n = 8) and 9% (n = 6), respectively, for both cations at the 30 μg L^-1 level. The device was successfully applied to the simultaneous determination of Pb(II) and Cd(II) in a honey sample.