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Fluorescence Lifetime Macro Imager for Biomedical Applications
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Fluorescence lifetime-based sensing and imaging.

Henryk Szmacinski1, Joseph R Lakowicz1

  • 1Center of Fluorescence Spectroscopy, Department of Biological Chemistry, University of Maryland, School of Medicine, 108 N. Greene St., Baltimore, MD 21201, USA.

Sensors and Actuators. B, Chemical
|April 19, 2021
PubMed
Summary
This summary is machine-generated.

Time-resolved fluorescence spectroscopy offers a powerful method for chemical sensing, providing quantitative analysis independent of probe intensity. This technique enables the detection of various analytes and has potential applications in clinical and environmental settings.

Keywords:
Lifetime-based sensingTime-resolved fluorescence spectroscopy

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Area of Science:

  • Biochemistry
  • Biophysics
  • Chemical Physics
  • Analytical Chemistry

Background:

  • Time-resolved fluorescence spectroscopy is primarily a research tool.
  • Intensity-based sensing methods have limitations.
  • Lifetime-based sensing offers advantages for quantitative analysis.

Purpose of the Study:

  • To highlight the potential of time-resolved fluorescence spectroscopy for chemical sensing.
  • To discuss the advantages of lifetime-based sensing over intensity-based methods.
  • To explore applications in various fields.

Main Methods:

  • Utilizing the collisional quenching mechanism for analyte detection (e.g., oxygen, halides).
  • Developing lifetime probes for analyte recognition (e.g., Ca2+, Mg2+, K+, pH).
  • Employing phase-modulation instrumentation and fluorescence lifetime imaging microscopy (FLIM).

Main Results:

  • Lifetime-based sensing is independent of total probe intensity, enabling quantitative measurements.
  • The method allows for the detection of analytes without direct probes, such as glucose.
  • FLIM provides chemical imaging capabilities within microscopy.

Conclusions:

  • Time-resolved fluorescence spectroscopy is a versatile tool for chemical sensing with broad applicability.
  • Advancements in instrumentation and probes facilitate migration to clinical, environmental, and industrial applications.
  • Fluorescence lifetime imaging microscopy offers new possibilities for chemical imaging in biological systems.