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Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing.

Iris Haberkorn1, Lya Siegenthaler1, Leandro Buchmann2

  • 1ETH Zürich, Laboratory of Sustainable Food Processing, Institute of Food, Nutrition and Health, Schmelzbergstrasse 9, 8092 Zürich, Switzerland.

Biotechnology Advances
|May 28, 2021
PubMed
Summary
This summary is machine-generated.

Nanosecond pulsed electric field (nsPEF) processing enhances single-cell bioconversion by inducing sublethal stress and affecting the plasma membrane. Understanding these mechanisms is key to optimizing nsPEF applications in various bio-based industries.

Keywords:
Bioconversion efficiency enhancementCompound stimulationGrowth stimulationPulsed electric fieldSingle-cells

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Area of Science:

  • Biotechnology
  • Biophysics
  • Cellular Biology

Background:

  • Nanosecond pulsed electric field (nsPEF) processing is a physical method to improve single-cell bioconversion efficiency and intracellular compound production.
  • Applications span pharmaceuticals, medicine, food, and feed production, but underlying mechanisms remain unclear.
  • A deeper understanding is crucial for process control, broader application, and industrial scale-up.

Purpose of the Study:

  • To elucidate the electrotechnological and metabolic fundamentals of nsPEF processing in the bio-based domain.
  • To critically evaluate the pathways responsible for enhanced single-cell bioconversion efficiency.
  • To provide best practice guidelines for nsPEF documentation to improve knowledge transfer.

Main Methods:

  • Evaluation of nsPEF effects on cellular growth states, focusing on the early to mid-exponential phase.
  • Analysis of combined effects including transient intracellular stress, sublethal stress induction, and plasma membrane alterations.
  • Investigation of cellular responses involving cytosolic Ca2+ hubs and reactive oxygen species (ROS) formation.

Main Results:

  • nsPEF treatment during the early to mid-exponential growth phase is critical for enhancing bioconversion efficiency.
  • Enhanced efficiency results from a combination of intracellular stress and plasma membrane effects.
  • Cellular responses involve transient cytosolic Ca2+ hubs and ROS formation, activating specific pathways.

Conclusions:

  • Understanding nsPEF mechanisms is vital for optimizing bioconversion efficiency and expanding its industrial use.
  • Standardized reporting and process control are essential for the successful scale-up of nsPEF technology.
  • Further research into specific cellular responses will facilitate broader application in biorefineries.