Pulsed Electric Fields for Emerging Single-Cell Bioprocessing in Food Applications: Electropermeabilization...

Area of Science:

• Food Science and Technology
• Biotechnology
• Electrochemistry

Background:

• Single-cell bioprocessing is crucial for the food industry.
• Pulsed electric fields (PEF) are an emerging technology with potential in food applications.
• Understanding electropermeabilization mechanisms is key to optimizing PEF.

Purpose of the Study:

• To review pulsed electric fields (PEF) for single-cell food bioprocessing.
• To connect electropermeabilization mechanisms with practical food applications.
• To provide a framework for designing PEF processes in the food sector.

Main Methods:

• Literature review of PEF applications in food bioprocessing.
• Analysis of electropermeabilization mechanisms.
• Development of a PEF process design framework.

Main Results:

• PEF is a viable platform for single-cell bioprocessing in food.
• Electropermeabilization is influenced by electrical, cellular, and system parameters.
• Cell density and organism choice significantly impact PEF outcomes.

Conclusions:

• A holistic approach linking mechanistic understanding and practical application enhances PEF scalability and reproducibility.
• PEF offers a promising avenue for advanced food bioprocessing.
• Further research can optimize PEF for diverse food applications.