Related Experiment Video
Updated: Nov 4, 2025
10:25
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
4.9K
Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe
Tadanobu Takahashi1, Yuuki Kurebayashi2, Tadamune Otsubo3
1Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan. takahasi@u-shizuoka-ken.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2021
Summary
Researchers developed a simple fluorescence imaging method for virus-infected cells. This technique visualizes viral neuraminidase activity without cell fixation or antiviral antibodies, aiding in virus detection and isolation.
Area of Science:
- Virology
- Cell Biology
- Biochemistry
Background:
- Traditional visualization of virus-infected cells relies on immunostaining, requiring cell fixation and antiviral antibodies.
- Influenza A, Influenza B, and certain paramyxoviruses express surface enzymes with sialidase activity.
Purpose of the Study:
- To establish a straightforward fluorescence (FL) imaging method for detecting virus-infected cells.
- To enable visualization without cell fixation or the use of antiviral antibodies.
Main Methods:
- Utilized a previously developed sialidase fluorogenic probe.
- Visualized the sialidase activity of viral neuraminidase or hemagglutinin-neuraminidase on infected cell surfaces.
- Applied the method for histochemical FL imaging.
Main Results:
- Successfully demonstrated fluorescence imaging of cells infected with influenza A, influenza B, and some paramyxoviruses.
- The method does not require cell fixation or antiviral antibodies.
- The probe effectively visualizes viral sialidase activity.
Conclusions:
- A simple and effective fluorescence imaging technique for virus-infected cells was established.
- This method is applicable for virus isolation and the detection of influenza viruses resistant to sialidase inhibitors.
Keywords:
Drug resistanceFluorescence imagingImaging probeInfected cellsInfluenza virusNeuraminidaseParamyxovirusSialidaseVirus isolation
