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Sialidase Activity Imaging of Different Organs with BTP3-Neu5Ac
Shota Hata1, Azliza Mad Anuar1,2, Yuuki Kurebayashi1
1Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
Advances in Experimental Medicine and Biology
|March 31, 2026
Summary
This study introduces BTP3-Neu5Ac for imaging sialidase activity, revealing its role in brain function, insulin secretion, and elastin production. Sialidase activity is crucial for cognitive function and neurotransmission regulation.
Area of Science:
- Biochemistry
- Neuroscience
- Pharmacology
Background:
- Sialidases are enzymes involved in various biological processes.
- Understanding sialidase distribution and function is crucial for therapeutic development.
Purpose of the Study:
- To explore the development and applications of BTP3-Neu5Ac for imaging sialidase activity.
- To elucidate the functional roles of sialidase in the brain, pancreas, and skin.
Main Methods:
- Development of the sialidase-imaging probe BTP3-Neu5Ac.
- Real-time imaging of sialidase activity in brain tissue.
- Investigating the effects of sialidase inhibition (DANA) on insulin secretion.
- Analyzing sialidase isozyme NEU2's role in skin elastin production.
Main Results:
- High sialidase activity observed in brain white matter and hippocampal glutamatergic nerve terminals.
- Sialidase activity rapidly increases with neural activity, modulating excitatory neurotransmission.
- Sialidase inhibitor DANA promotes insulin secretion without causing hypoglycemia, suggesting diabetes treatment potential.
- Sialidase isozyme NEU2 enhances elastin production in the skin.
Conclusions:
- BTP3-Neu5Ac is a valuable tool for mapping sialidase activity in vivo.
- Sialidase plays significant roles in cognitive function, neurotransmission, and metabolic regulation.
- Targeting sialidase offers potential therapeutic strategies for neurological disorders, diabetes, and skin aging.
