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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Nov 4, 2025

In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice

In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice

Published on: August 10, 2018

In Vivo Flow Cytometry.

Xi Zhu¹, Qi Liu¹, Yuting Fu¹

¹State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.

Advances in Experimental Medicine and Biology

|May 30, 2021

Summary

This summary is machine-generated.

In vivo flow cytometry (IVFC) enables real-time cell monitoring in peripheral blood without blood draws. Recent advancements in microscopy have expanded its applications beyond circulating cells, enhancing biomedical research capabilities.

Keywords:

Biomedical application Circulating tumor cell Fluorescence detection In vivo flow cytometry Label-free flow cytometry Photoacoustic detection

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Published on: July 30, 2020

Area of Science:

Biomedical Engineering
Optical Imaging
Cellular Biology

Background:

In vivo flow cytometry (IVFC) was initially developed for detecting circulating cells in mouse models.
Significant advancements in fluorescence, photoacoustic, and multiphoton microscopy have propelled the IVFC field forward.
The scope of IVFC applications has broadened beyond monitoring circulating cells.

Purpose of the Study:

To provide a comprehensive overview of the technical aspects and key applications of in vivo flow cytometry.
To detail the principles, labeling strategies, sensitivity, and biomedical uses of IVFC.
To discuss future research directions and emerging trends in IVFC technology.

Main Methods:

Focus on fluorescence-based IVFC for superficial vessel monitoring.
Highlight photoacoustic-based IVFC for label-free deep vessel imaging.
Discuss advancements in microscopy techniques enhancing IVFC capabilities.

Main Results:

IVFC offers real-time, non-invasive monitoring of cells in peripheral blood.
Fluorescence and photoacoustic IVFC are the most prevalent methods in biomedical research.
Photoacoustic methods excel in deep vessel imaging, while fluorescence methods are suited for superficial vessels.

Conclusions:

IVFC is a rapidly evolving technology with diverse biomedical applications.
Continued development in microscopy and labeling strategies will further enhance IVFC sensitivity and scope.
Future research should explore novel applications and address challenges in deep tissue imaging and quantitative analysis.