Related Experiment Video
Updated: Nov 4, 2025

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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS
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Flow Cytometry Instrument Setting as a Crucial Checkpoint for Optimal T-Cell Analysis and Sorting
Achille Anselmo1, Federico Simone Colombo2
1Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy. achille.anselmo@humanitasresearch.it.
Methods in Molecular Biology (Clifton, N.J.)
|May 30, 2021
Abstract:
Although flow cytometry and cell sorting are widely used by immunologists both for basic and translation research, many aspects of these techniques should be optimized to obtain reproducible and meaningful data. In this chapter we provide some protocols and tips on instrument setting, multicolor panel design and T-cell immunophenotyping and proliferation assay.

