Monoclonal antibodies to blood group antigens

To obtain haemagglutinating monoclonal antibodies as potential blood typing reagents 10 fusions were performed between mouse myeloma cells and spleen cells from mice immunised with either blood group substances or human red blood cells. Nineteen hybridomas with anti-A specificity and 10 with anti-B specificity were generated, Three were selected for further investigations. Some of these hybridoma clones produce and secrete both IgM and IgG.