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Updated: Nov 2, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues.

Ruili Feng^1,2,3, Dongyue Wang^1,2,3, Yanlu Chen^1,2,3

¹Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.

|June 7, 2021

Summary

This summary is machine-generated.

We present a protocol for a versatile tiling light sheet microscope, enabling high-resolution imaging of cleared tissues. This advanced microscopy technique offers cellular to subcellular detail for biological research.

Keywords:

Microscopy Neuroscience

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Area of Science:

Biomedical Engineering
Microscopy
Optical Imaging

Background:

Advanced microscopy techniques are crucial for visualizing biological structures.
Conventional light sheet microscopy has limitations in spatial resolution and optical sectioning.
Imaging cleared tissues requires specialized microscopy for detailed analysis.

Purpose of the Study:

To describe a protocol for constructing, calibrating, and operating a versatile tiling light sheet microscope.
To achieve cellular to subcellular spatial resolution in imaging cleared tissues.
To improve upon conventional light sheet microscopy for enhanced imaging capabilities.

Main Methods:

Development of a versatile tiling light sheet microscope.
Utilizing adjustable tiling light sheets for improved imaging.
Implementing semiautomatic alignment via phase modulation of illumination light.
Ensuring compatibility with all tissue clearing methods.

Main Results:

The developed microscope achieves higher spatial resolution than conventional methods.
Superior optical sectioning ability is demonstrated.
Successful imaging of cleared tissues at cellular to subcellular resolution.

Conclusions:

The protocol provides a method for a versatile tiling light sheet microscope.
This technique enhances imaging capabilities for cleared tissues.
The microscope offers a valuable tool for high-resolution biological imaging.