Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Leveraging factors that control alveolar epithelial cell fate enables large-scale expansion for lung tissue engineering.

The Journal of clinical investigation·2026

Same author

Better reporting is better science: Community-defined minimal reporting requirements for light microscopy.

The Journal of cell biology·2026

Same author

Transmembrane proteins mediate basal complex assembly and individual daughter cell formation in malaria parasites.

Science advances·2026

Same author

HIV-producing resting T cells monopolize virus production, associated PANoptosis, and persistence in lymphoid tissue.

bioRxiv : the preprint server for biology·2026

Same author

Interferon lambda signaling to maternal dendritic cells protects against congenital Zika virus infection.

bioRxiv : the preprint server for biology·2026

Same author

Highly multiplexed 3D profiling of cell states and immune niches in human tumors.

Nature methods·2025

Search research articles

Related Experiment Video

Updated: Nov 2, 2025

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

Best practices and tools for reporting reproducible fluorescence microscopy methods.

Paula Montero Llopis¹, Rebecca A Senft², Tim J Ross-Elliott³

¹MicRoN Core, Harvard Medical School, Boston, MA, USA. paula_monterollopis@hms.harvard.edu.

|June 8, 2021

Summary

Accurate reporting of fluorescence microscopy methods is crucial for reproducible biomedical research. This study provides guidelines to improve microscopy metadata reporting and enhance image-based science quality.

More Related Videos

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Published on: March 19, 2014

Related Experiment Videos

Last Updated: Nov 2, 2025

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Published on: March 19, 2014

Area of Science:

Biomedical research
Microscopy
Image analysis

Background:

Fluorescence microscopy is widely used in biomedical research.
Current methods reporting is inconsistent and undervalued.
Inconsistent reporting hinders data interpretation and reproducibility.

Purpose of the Study:

To emphasize the importance of accurate microscopy methods reporting.
To educate researchers on the impact of microscopy metadata.
To improve the quality, rigor, and reproducibility of image-based science.

Main Methods:

Providing comprehensive guidelines for reporting microscopy methods.
Offering resources for common fluorescence light microscopy modalities.
Focusing on metadata crucial for data interpretation.

Main Results:

Development of guidelines for accurate microscopy reporting.
Educational resources to improve researcher understanding.
Standardization of reporting for fluorescence microscopy.

Conclusions:

Improved microscopy reporting enhances data interpretation.
Accurate metadata is essential for rigorous image-based science.
Standardized reporting will advance the field of fluorescence microscopy.