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Updated: Nov 2, 2025

Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice
Published on: August 13, 2020
[IMPROVING THE PERFUSION FLOW OF BLOOD PUMPS]
N Khodeli1, Z Chkhaidze1, O Shengelia1
1I. Javakhishvili Tbilisi State Universitety, Institute of Morphology, Georgia.
Abstract:
Research in recent years has shown that pulsating flow during cardiopulmonary bypass can prevent the development of a number of perfusion complications inherent in continuous blood flow. On the other hand, attempts to pulsate with roller and centrifugal pumps, which are structurally DC pumps, are fraught with complications. However, these pumps are clinically tested and are successfully used for the implementation of direct blood flow. The aim of the study was to develop and create a preclinical apparatus that transforms the flow of DC pumps into a pulsating one. We have developed an electronically controlled device (pulsator) that transforms the direct blood flow generated by roller or centrifugal pumps into a pulsating flow synchronized by the cardiogram. The pulsator was tested on the bench in various load modes. Cardiosynchronized pulsating blood flow with circulatory support is able to effectively relieve the ventricular myocardium both in volume and pressure. The pulsator can be easily and easily installed on the operating table or on the perfusion device stand. The ability to control the pulsation frequency, systolic-diastolic duration, clear cardiosynchronization and the ability to control the onset and termination of the impulse can provide unloading of the myocardium in volume and pressure in experiments on animals in the mode of auxiliary circulation.
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