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Related Experiment Video

Updated: Nov 2, 2025

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

Caged lipids for subcellular manipulation.

Scotland Farley¹, Aurélien Laguerre¹, Carsten Schultz¹

¹Dept. Chemical Physiology & Biochemistry, Oregon Health and Science University (OHSU), Portland, OR, USA.

Current Opinion in Chemical Biology

|June 13, 2021

Summary

This summary is machine-generated.

Researchers developed light-activated strategies to control lipid levels within live cells. These methods enable precise manipulation of lipid localization and activation for advanced cellular studies.

Keywords:

Fluorescence Lipid transport Photoactivation Signaling

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Last Updated: Nov 2, 2025

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

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Published on: February 9, 2018

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

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Published on: January 11, 2017

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Area of Science:

Cell Biology
Biochemistry
Chemical Biology

Background:

Lipid metabolism is crucial for cellular function.
Precise control over lipid localization and activity is essential for studying cellular processes.
Existing methods for lipid manipulation lack spatiotemporal control.

Purpose of the Study:

To present novel light-inducible strategies for manipulating lipid levels in live cells.
To explore the use of photoremovable protecting groups for subcellular lipid control.
To highlight advancements in organelle-specific lipid targeting.

Main Methods:

Development and application of photoremovable protecting groups (caging groups) for lipids.
Utilizing light to trigger lipid deprotection and activation at specific subcellular locations.
Designing caging groups with chromatic orthogonality for multi-channel control.

Main Results:

Demonstrated successful light-induced manipulation of lipid levels in live cells.
Achieved subcellularly restricted localization and activation of lipids using photoremovable protecting groups.
Showcased organelle targeting of caged lipids.

Conclusions:

Light-based strategies offer precise spatiotemporal control over cellular lipid metabolism.
Photoremovable protecting groups are effective tools for studying lipid function in situ.
Chromatic orthogonality of caging groups opens new avenues for complex lipid manipulation studies.