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[A new approach to selecting antibacterial preparations for treating patients with chronic urological infections]
Abstract:
The oral administration of microflora, isolated from the urinary tract or feces of urological patients, into the abacterial digestive tract of mice kept under the conditions of germ-free environment leads to the formation of the donors' microflora in the intestine of the recipient animals. Such recipient mice can be used as models for the rapid and highly reliable determination of the sensitivity of microflora in the patient's intestine and urinary tract to different antimicrobial preparations. In different patients such microflora, though similar in its qualitative and quantitative composition, differs in sensitivity to different antimicrobial preparations and their combinations. For the effective treatment of patients with chronic urinary tract infections a preliminary in vivo determination of the sensitivity of intestinal and urinary microflora to different combinations of antimicrobial preparations, carried out with the use of germ-free techniques, is necessary.
Insights
Germ-free mice models effectively mimic patient microflora for antimicrobial sensitivity testing. This allows for personalized treatment strategies for chronic urinary tract infections.
Area of Science:
- Microbiology
- Urology
- Animal Models
Background:
- Chronic urinary tract infections (UTIs) pose treatment challenges.
- Microflora composition varies between patients, impacting treatment efficacy.
- Standard antimicrobial sensitivity testing may not fully represent in vivo conditions.
Purpose of the Study:
- To develop a reliable animal model for assessing microflora antimicrobial sensitivity.
- To investigate the variability of microflora sensitivity to antimicrobials in urological patients.
- To establish a method for optimizing antimicrobial treatment for chronic UTIs.
Main Methods:
- Oral administration of patient-derived microflora (urine/feces) into germ-free mice.
- Cultivation of donor microflora in the recipient mice's digestive tract.
- In vivo determination of microflora sensitivity to various antimicrobial agents and combinations.
Main Results:
- Germ-free mice successfully harbored microflora from urological patients.
- The developed model allowed for rapid and reliable assessment of microflora sensitivity.
- Significant differences in antimicrobial sensitivity were observed among patient-derived microflora.
Conclusions:
- Germ-free mouse models are valuable for predicting antimicrobial efficacy.
- In vivo sensitivity testing is crucial for effective treatment of chronic UTIs.
- Personalized antimicrobial selection based on this model can improve patient outcomes.