[Research progress of microextraction by packed sorbent and its application in microvolume sample extraction].

Area of Science:

• Analytical Chemistry
• Sample Preparation Technologies
• Microextraction Techniques

Context:

• Traditional sample preparation methods are often time-consuming and lack miniaturization and automation.
• The demand for rapid, efficient, and green analytical methods necessitates advanced sample preparation techniques.
• Microextraction using a packed sorbent (MEPS) has emerged as a promising alternative to conventional methods.

Purpose:

• To introduce and describe Microextraction using a packed sorbent (MEPS) as a novel sample preparation technology.
• To detail the components, operation, and optimization parameters of MEPS.
• To highlight the versatility of MEPS with various sorbent types and its application in different matrices.

Summary:

• MEPS utilizes a miniaturized solid-phase extraction device integrated into a microsyringe for sample preparation.
• The process involves conditioning, loading, washing, and elution steps, with optimization of parameters like flow rate, sample volume, and sorbent type.
• MEPS is compatible with various commercial and non-commercial sorbents and can be automated for increased efficiency.

Impact:

• MEPS significantly improves analysis efficiency, data quality, and reduces sample and solvent consumption.
• Its adaptability to complex matrices like biological fluids, environmental, and food samples is demonstrated through online LC-MS and GC-MS assays.
• MEPS is poised for wider adoption in bio-matrix analysis and field-based sample pretreatment due to its speed and small sample size requirements.