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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Author Correction: A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells

Yulei Zhao1,2, Kathrin Tyrishkin1, Calvin Sjaarda3,4

  • 1Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.

Scientific Reports
|July 9, 2021
PubMed
Abstract

No abstract available in PubMed .

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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