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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

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Published on: May 3, 2011

AIE + ESIPT activity-based NIR Cu²⁺ sensor with dye participated binding strategy.

Jie Jiang¹, Haifeng Sun¹, Yanlei Hu¹

¹School of Chemistry and Chemical Engineering, Key Laboratory of the Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan 250100, China. jiejiang@sdu.edu.cn.

Chemical Communications (Cambridge, England)

|July 13, 2021

Summary

This summary is machine-generated.

Researchers developed a novel fluorescent probe for detecting copper ions (Cu2+). This probe uses chelation to trigger a near-infrared emission, enabling ratiometric imaging in living cells.

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A Polyaniline-based Sensor of Nucleic Acids

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Area of Science:

Analytical Chemistry
Biophysical Chemistry
Materials Science

Background:

Copper ions (Cu2+) play crucial roles in biological processes.
Dysregulation of Cu2+ is linked to various diseases.
Sensitive and specific detection methods for Cu2+ are needed.

Purpose of the Study:

To synthesize a novel activity-based fluorescent probe for Cu2+.
To investigate the probe's mechanism based on chelation-triggered hydrolysis.
To evaluate the probe's utility for ratiometric imaging of Cu2+ in live cells.

Main Methods:

Synthesis of a multidentate fluorescent probe.
Investigation of probe-Cu2+ interaction via chelation.
Characterization of probe hydrolysis and emission properties (AIE + ESIPT).
Ratiometric imaging experiments in living HeLa cells.

Main Results:

A novel activity-based fluorescent probe for Cu2+ was successfully synthesized.
The probe exhibits specific chelation with Cu2+, triggering hydrolysis.
The hydrolysis releases a near-infrared emission with Aggregation-Induced Emission (AIE) and Excited-State Intramolecular Proton Transfer (ESIPT) properties.
The probe demonstrated effective ratiometric imaging of Cu2+ in living HeLa cells.

Conclusions:

The developed probe offers a sensitive and specific method for Cu2+ detection.
The probe's unique properties enable ratiometric imaging in biological systems.
This work provides a valuable tool for studying Cu2+ in cellular environments.