Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

5.0K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.0K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

8.2K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.2K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

8.3K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
8.3K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.0K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.0K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

7.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.9K
Phagocytosis00:41

Phagocytosis

88.2K
Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis (“cellular eating”) is one of three major types of endocytosis. Cells use phagocytosis to take in large objects—such as other cells (or their debris), bacteria, and even viruses.
88.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Characteristics and Influencing Factors of Depressive Symptoms in Patients With Vascular Cognitive Impairment.

Actas espanolas de psiquiatria·2026
Same author

Holistic genome assembly and analysis of the <i>Tremella fuciformis</i> interaction community uncovers intergenomic insights beyond dual genomes.

IMA fungus·2026
Same author

Liposomal mitoxantrone plus tislelizumab in patients with relapsed or refractory extranodal natural killer/T-cell lymphoma: a phase 1b/2 trial.

Nature communications·2026
Same author

SLC22A4 as a candidate regulator linking immunity and ferroptosis in septic shock.

Molecular immunology·2026
Same author

Integrating ex vivo drug sensitivity and genetic mutation analysis improves prediction of chemotherapy response in acute myeloid leukemia.

Functional & integrative genomics·2026
Same author

Immune-Genomic Evolution in AML Spontaneous Remission: A 66-Patient Pooled Analysis and Longitudinal Clonal Tracking.

Cancers·2026
Same journal

Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

Advances in experimental medicine and biology·2026
Same journal

Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

Advances in experimental medicine and biology·2026
Same journal

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026
Same journal

Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

Advances in experimental medicine and biology·2026
Same journal

PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

Advances in experimental medicine and biology·2026
Same journal

Dynamic Assemblies in Genome Maintenance.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Oct 29, 2025

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

759

Autophagosome Trafficking.

Jingjing Ye1, Ming Zheng2

  • 1Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, China.

Advances in Experimental Medicine and Biology
|July 14, 2021
PubMed
Summary
This summary is machine-generated.

Autophagy is a cellular recycling process. Understanding autophagosome trafficking mechanisms is crucial for cellular health, involving cytoskeletal and motor proteins.

More Related Videos

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
09:00

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

18.7K
Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein
07:20

Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

2.4K

Related Experiment Videos

Last Updated: Oct 29, 2025

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

759
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
09:00

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

18.7K
Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein
07:20

Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

2.4K

Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Autophagy is a fundamental cellular process for degrading and recycling cellular components.
  • Autophagosome trafficking and fusion with lysosomes are critical for completing autophagic degradation.
  • The transport mechanisms of autophagosomes, especially in specialized cells, are not fully understood.

Purpose of the Study:

  • To elucidate the processes and mechanisms governing autophagosome trafficking to lysosomes during autophagy.
  • To highlight the importance of understanding autophagosome transport in various cell types.

Main Methods:

  • Investigating the roles of cytoskeletal systems (actin, microtubules) and motor proteins (myosins, dynein-dynactin) in organelle trafficking.
  • Examining regulatory factors and reagents that impact autophagosome transport.
  • Utilizing microtubule polymerization inhibitors, F-actin-depolymerizing drugs, and motor protein regulators as experimental tools.

Main Results:

  • Autophagosome trafficking relies on conserved organelle transport machinery, including cytoskeletal elements and motor proteins.
  • Specific reagents targeting microtubules, F-actin, and motor proteins disrupt autophagosome trafficking.
  • The study identifies key components and factors influencing the movement of autophagosomes.

Conclusions:

  • Autophagosome trafficking is a complex process mediated by cytoskeletal networks and motor proteins.
  • Disrupting these components significantly impacts autophagosome transport, offering insights into autophagy regulation.
  • Further research into autophagosome trafficking mechanisms is essential for understanding cellular homeostasis and disease.