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Updated: Oct 25, 2025
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Carbon-doped WO3 electrochemical aptasensor based on Box-Behnken strategy for highly-sensitive detection of
Jialing Song1, Xuanhao Lin2, Nan Jiang3
1College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China; National University of Singapore, Department of Chemistry, 3 Science Drive 3, Singapore 117543, Singapore.
Abstract:
Aptamer has been proved to be an important probe for antibiotic detection. Here, the electrical signal was doubly amplified by the synergistic effect of C-WO3 and AuNPs. The probe structure has a specific recognition effect on tetracycline, which improves the selectivity and anti-interference of the sensor. With the assistance of BBD strategy, the experimental errors of the C-WO3@AuNPs aptasensor were reduced and the best conditions for its preparation were obtained. This was conducive to obtain the best electrical signal transmission capacity of the electrode, greatly improved the sensor sensitivity. Under this mechanism, the antibiotic sensor achieved a low detection range (0.1 nM-100 nM) and a low detection limit (4.8 × 10-2 nM). The sensor showed excellent selectivity even in the presence of coexisting pollutants. This work explored the mechanism of charge change and demonstrated the role of probes in antibiotic sensing, providing important prospects of future applications in electrochemical sensors.
