Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

934
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
934
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

2.1K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
2.1K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.2K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.2K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.5K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.5K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

2.1K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
2.1K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

352
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
352

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tracking the Early Hydration Reaction of Cementitious Calcium Silicate Hydrate via DNP-Enhanced Solid-State NMR.

Journal of the American Chemical Society·2026
Same author

In Situ Light-Induced Degradation of Hybrid Perovskites by NMR Spectroscopy.

Journal of the American Chemical Society·2026
Same author

Halide Mixing Determines the Organic Structure in 2D Layered Perovskites.

The journal of physical chemistry letters·2026
Same author

Generation of Membrane-Damaging hIAPP Oligomers via Direct Interaction with DOPC/DOPS Nanodiscs.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Rapid Assignment of Chemical Shifts From Crystal Structures in Solid-State NMR.

Angewandte Chemie (International ed. in English)·2026
Same author

Surface-Only Nuclear Magnetic Resonance Spectroscopy by Dynamic Nuclear Polarization and <sup>2</sup>H-Dephasing.

Journal of the American Chemical Society·2026
Same journal

Ptychography at all wavelengths.

Nature reviews. Methods primers·2026
Same journal

Droplet-based bioprinting.

Nature reviews. Methods primers·2026
Same journal

Laser capture microdissection.

Nature reviews. Methods primers·2026
Same journal

Extracellular vesicle analysis.

Nature reviews. Methods primers·2026
Same journal

In vivo microelectrode arrays for neuroscience.

Nature reviews. Methods primers·2026
Same journal

Light-based vat-polymerization bioprinting.

Nature reviews. Methods primers·2025
See all related articles

Related Experiment Video

Updated: Oct 25, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.6K

Solid-state NMR spectroscopy.

Bernd Reif1, Sharon E Ashbrook2, Lyndon Emsley3

  • 1Technische Universität München, Department Chemie, Lichtenbergstr. 4, D-85747 Garching, Germany.

Nature Reviews. Methods Primers
|August 9, 2021
PubMed
Summary
This summary is machine-generated.

Solid-state nuclear magnetic resonance (NMR) spectroscopy provides atomic-level insights into solid and semi-solid structures and dynamics. This primer details magic-angle spinning (MAS) NMR methods for diverse materials and future advancements.

More Related Videos

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.3K

Related Experiment Videos

Last Updated: Oct 25, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.6K
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.3K

Area of Science:

  • Chemistry
  • Physics
  • Materials Science
  • Biochemistry

Background:

  • Solid-state nuclear magnetic resonance (NMR) spectroscopy is a powerful atomic-level technique.
  • It is used to determine the chemical structure, 3D structure, and dynamics of solids and semi-solids.
  • While fundamental principles align with liquid-state NMR, solid-state applications require specialized techniques due to anisotropic interactions.

Purpose of the Study:

  • To summarize the basic principles of solid-state NMR spectroscopy.
  • To describe common magic-angle spinning (MAS) NMR experiments and data analysis for various solid systems.
  • To highlight recent advancements and future directions in solid-state NMR.

Main Methods:

  • The primer explains fundamental nuclear spin interactions and responses to magnetic fields and radiofrequency pulses.
  • Magic-angle spinning (MAS) is detailed as a key technique for high-resolution solid-state NMR spectra.
  • Various sensitivity-enhancement approaches, including 1H-detected fast MAS, dynamic nuclear polarization, and ultrahigh magnetic field experiments, are discussed.

Main Results:

  • The text outlines common MAS NMR experiments and data analysis for biological macromolecules, organic materials, and inorganic solids.
  • Recent applications in biological and materials chemistry are highlighted.
  • Current limitations of solid-state NMR are discussed, alongside potential future developments.

Conclusions:

  • Solid-state NMR is a versatile technique for atomic-level structural and dynamical analysis of diverse solid materials.
  • Advancements in MAS NMR, sensitivity enhancement, and ultrahigh magnetic fields are expanding its capabilities.
  • Future developments promise to further broaden the applications of solid-state NMR in chemistry and materials science.