Comparison of Different Dentin Deproteinizing Agents on the Shear Bond Strength of Resin-bonded Dentin

To assess and analyze the resin-bonded dentin in terms of shear bond strength after using deproteinizing agents 5% sodium hypochlorite, 10% sodium hypochlorite, and bromelain.

Overall 140 permanent and intact human premolars were split into four groups, three experimental groups and one control group. In all four groups, the occlusal surface of the teeth was wet ground to expose superficial dentin. In group I, teeth were etched and deproteinized with 5% sodium hypochlorite. In group II, teeth were etched and deproteinized with 10% sodium hypochlorite. In group III, teeth were etched and bromelain was used to deproteinize. In group IV, teeth were etched and no deproteinization was being performed and simultaneous fulfillment of the resin composite and later inserted into the plastic tube and polymerized with light. Samples were stored at 37°C for 24 hours and the later samples were transferred to the universal testing machines to shear bond strength analysis at a speed of 0.5 mm/minute.

The outcome of the bond strength was significantly influenced by the application of bromelain enzymes. A remarkable difference was observed between the shear bond strength of sodium hypochlorite (5 and 10%) and in the bromelain enzyme-treated group. Group III showed better results than group I and group II.

This study concluded that bromelain shear has the maximum value for shear bond strength. Bond strength improved because of removal of unsupported collagen fiber with bromelain enzyme after acid etching.

Natural pineapple enzyme, i.e., bromelain improves bond strength by removal of unsupported collagen fiber. Hence, it is completely safe to use.

Khan R, Sharma N, Garg Y, et al. Comparison of Different Dentin Deproteinizing Agents on the Shear Bond Strength of Resin-bonded Dentin. Int J Clin Pediatr Dent 2020;13(S-1):S69-S77.