Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation and Reactions of Thiols02:33

Preparation and Reactions of Thiols

6.9K
Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.
6.9K
Preparation and Reactions of Sulfides02:26

Preparation and Reactions of Sulfides

5.2K
Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
5.2K
Preparation of Alkynes: Dehydrohalogenation02:34

Preparation of Alkynes: Dehydrohalogenation

16.8K
Introduction
Alkynes can be prepared by dehydrohalogenation of vicinal or geminal dihalides in the presence of a strong base like sodium amide in liquid ammonia. The reaction proceeds with the loss of two equivalents of hydrogen halide (HX) via two successive E2 elimination reactions.
16.8K
Preparation of Aldehydes and Ketones from Nitriles and Carboxylic Acids01:24

Preparation of Aldehydes and Ketones from Nitriles and Carboxylic Acids

3.9K
Although it is possible to reduce a carboxylic acid to an aldehyde, strong reducing agents, like lithium aluminum hydride (LAH), prohibit a controlled reduction, instead causing the generated aldehyde to instantly over-reduce to a primary alcohol.
Reducing carboxylic acid derivatives like acyl chlorides (RCOCl), esters (RCO2R′), and nitriles (RCN) using milder aluminum hydride agents like lithium tri-tert-butoxyaluminum hydride [LiAlH(O-t-Bu)3] and diisobutylaluminum hydride [DIBAL-H]...
3.9K
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

565
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
565
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

7.5K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic ROS Generation via Core-Shell Nanostructures with Increased Lattice Microstrain Combined with Single-Atom Catalysis for Enhanced Tumor Suppression.

ACS applied materials & interfaces·2024
Same author

Establish and validate the reliability of predictive models in bone mineral density by deep learning as examination tool for women.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2023
Same author

The Competition of Yin and Yang: Exploring the Role of Wild-Type and Mutant p53 in Tumor Progression.

Biomedicines·2023
Same author

Microfluidic Synthesis of -NH<sub>2</sub>- and -COOH-Functionalized Magnetite Nanoparticles.

Nanomaterials (Basel, Switzerland)·2022
Same author

P63 and P73 Activation in Cancers with p53 Mutation.

Biomedicines·2022
Same author

Screening of Specific and Common Pathways in Breast Cancer Cell Lines MCF-7 and MDA-MB-231 Treated with Chlorophyllides Composites.

Molecules (Basel, Switzerland)·2022
Same journal

Correction: Reis et al. Bioinks Enriched with ECM Components Obtained by Supercritical Extraction. <i>Biomolecules</i> 2022, <i>12</i>, 394.

Biomolecules·2026
Same journal

Correction: Kim, K.-H.; Yoo, B.C. Gintonin as a Lysophosphatidic Acid-Enriched GPCR Ligand System: Molecular Architecture and Receptor Pharmacology in <i>Panax ginseng</i>. <i>Biomolecules</i> 2026, <i>16</i>, 465.

Biomolecules·2026
Same journal

Correction: Bastyte et al. The Association of Vitamin D Receptor Gene Polymorphisms with Vitamin D, Total IgE, and Blood Eosinophils in Patients with Atopy. <i>Biomolecules</i> 2024, <i>14</i>, 212.

Biomolecules·2026
Same journal

AtHSPR Plays a Positive Role in Arabidopsis Resistance Against <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000 by Interacting with TOP1.

Biomolecules·2026
Same journal

CYTH4 Facilitates Renal Cell Carcinoma via Enhancing Proliferation and Likely Immune Evasion.

Biomolecules·2026
Same journal

Integrated Immune-Gut Profiling Identifies an Exploratory Pediatric Inflammatory Intestinal Profile Associated with Food-Specific IgG Reactivity.

Biomolecules·2026
See all related articles

Related Experiment Video

Updated: Oct 22, 2025

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
11:26

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

Published on: March 21, 2016

14.8K

Chlorophyllides: Preparation, Purification, and Application.

Yi-Ting Wang1, Chih-Hui Yang1,2,3, Keng-Shiang Huang4

  • 1Department of Biological Science and Technology, I-Shou University, Kaohsiung 82445, Taiwan.

Biomolecules
|August 27, 2021
PubMed
Summary
This summary is machine-generated.

Chlorophyllides, found in photosynthetic organisms, are vital compounds with diverse applications in food, medicine, and pharmaceuticals. This review details their definition, biosynthesis, purification, and biological activities.

Keywords:
chlorophyllasechlorophyllideschlorophylls

More Related Videos

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.3K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.4K

Related Experiment Videos

Last Updated: Oct 22, 2025

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
11:26

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

Published on: March 21, 2016

14.8K
Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
07:10

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Published on: February 3, 2023

1.3K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.4K

Area of Science:

  • Biochemistry
  • Photosynthesis Research

Background:

  • Chlorophyllides are derivatives of chlorophyll, essential in photosynthetic organisms.
  • They possess a characteristic tetrapyrrole structure with a central Mg ion.
  • Common types include chlorophyllides a, b, c, d, and f.

Purpose of the Study:

  • To provide a comprehensive review of chlorophyllides.
  • To detail their definition, biosynthesis, and purification methods.
  • To explore their diverse applications and biological activities.

Main Methods:

  • Literature review of chlorophyllide research.
  • Analysis of biosynthetic pathways.
  • Summary of purification techniques.
  • Compilation of application data.

Main Results:

  • Chlorophyllides are synthesized from various precursors, including protochlorophyllide a.
  • Chlorophyllide a can be interconverted into other chlorophyllide types (b, d, f).
  • Chlorophyllide c is synthesized from protochlorophyllide a or divinyl protochlorophyllide a.

Conclusions:

  • Chlorophyllides have significant applications in food, medicine, and pharmaceuticals.
  • They exhibit a wide range of biological activities, including antimicrobial and antiproliferative effects.
  • Their photosensitivity is relevant for sensor technology.