Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.5K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.5K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.5K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.5K
Hematopoiesis01:21

Hematopoiesis

7.2K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
7.2K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.3K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.3K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

5.6K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
5.6K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

2.0K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary omega-6 lipids promote post-injury aberrant bone formation in obesity.

The Journal of clinical investigation·2026
Same author

Genomic landscape of the human vaginal microbiome is linked to host genetics and population of origin.

Nature genetics·2026
Same author

Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.

Cell reports·2026
Same author

Multiomic Profiling of 85 iPSC Lines from Familial Dementia Reveals Cellular Diversity and Regulators of Organoid Development.

bioRxiv : the preprint server for biology·2026
Same author

MitoSAM-dependent lipoylation controls postnatal heart development via metabolic remodeling.

bioRxiv : the preprint server for biology·2026
Same author

Nitrogen metabolism profiling reveals cell state-specific pyrimidine synthesis pathway choice.

Nature metabolism·2026
Same journal

Psychological stress drives aging-like hematopoietic stem cell dysfunction through a brain-gut-bone marrow axis.

Cell stem cell·2026
Same journal

Human stem cell-based embryo model governance: Insights from Japan.

Cell stem cell·2026
Same journal

From reconstruction to intervention: Engineered organoids as living therapeutic depots.

Cell stem cell·2026
Same journal

Long-term lessons from MATCH01 macrophage therapy in cirrhosis.

Cell stem cell·2026
Same journal

Beyond apoptosis: LSC state dictates metabolic and anti-apoptotic vulnerabilities.

Cell stem cell·2026
Same journal

Outside the niche: Gut microbiota relay psychological stress to hematopoietic stem cell dysfunction.

Cell stem cell·2026
See all related articles

Related Experiment Video

Updated: Oct 22, 2025

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
08:53

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

15.5K

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.

Le Qi1, Misty S Martin-Sandoval1, Salma Merchant1

  • 1Children's Research Institute and the Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Cell Stem Cell
|August 27, 2021
PubMed
Summary
This summary is machine-generated.

Hematopoietic stem cells (HSCs) require aspartate synthesis for function. Aspartate availability limits HSC regeneration by impacting purine and asparagine synthesis.

Keywords:
asparagineaspartateelectron transport chainhematopoietic stem cellmetabolismmitochondriapurineregeneration

More Related Videos

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

18.9K
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

4.9K

Related Experiment Videos

Last Updated: Oct 22, 2025

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
08:53

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

15.5K
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

18.9K
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

4.9K

Area of Science:

  • Cellular metabolism
  • Hematopoiesis
  • Stem cell biology

Background:

  • Aspartate synthesis is crucial for cancer cell proliferation.
  • The role of aspartate in normal stem cell function remains largely unexplored.
  • Hematopoietic stem cells (HSCs) are critical for blood regeneration.

Purpose of the Study:

  • To investigate the dependence of hematopoietic stem cells (HSCs) on cell-autonomous aspartate synthesis.
  • To determine the metabolic pathways and downstream effectors influenced by aspartate in HSCs.
  • To elucidate the role of aspartate availability in HSC function during hematopoietic regeneration.

Main Methods:

  • Genetic manipulation of aspartate synthesis enzymes (Got1, Got2) and transporters (Glast) in mouse HSCs.
  • Assessment of HSC function and progenitor cell activity.
  • Isotope tracing to identify aspartate utilization pathways.
  • Pharmacological inhibition of downstream pathways (asparagine synthetase, purine synthesis).

Main Results:

  • Mouse HSCs rely entirely on cell-autonomous aspartate synthesis, which is upregulated upon activation.
  • Modulating aspartate levels via Glast overexpression or Got1/Got2 deletion significantly impacted HSC function.
  • Aspartate is essential for synthesizing asparagine and purines, both critical for HSC function.
  • Deletion of both Got1 and Got2 resulted in the complete loss of HSCs.

Conclusions:

  • HSC function during hematopoietic regeneration is limited by the availability of aspartate, purines, and asparagine.
  • Cell-autonomous aspartate synthesis is a key regulatory mechanism for HSCs.
  • Targeting aspartate metabolism offers potential therapeutic strategies for hematopoietic disorders.