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Common-path intrinsically achromatic optical diffraction tomography.

Piotr Zdańkowski1,2,3, Julianna Winnik1,2,4, Krzysztof Patorski1

  • 1Warsaw University of Technology, Institute of Micromechanics and Photonics, 8 Św. A. Boboli st., 02-525 Warsaw, Poland.

Biomedical Optics Express
|August 30, 2021
PubMed
Summary
This summary is machine-generated.

We developed a low-cost, compact optical diffraction tomography system that is immune to vibrations. Using partially coherent light sources significantly reduces noise and improves tomographic reconstruction fidelity.

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Area of Science:

  • Optical physics
  • Tomography
  • Interferometry

Background:

  • Traditional optical diffraction tomography systems can be complex, costly, and sensitive to environmental vibrations.
  • Achieving high-fidelity tomographic reconstructions often requires sophisticated optical setups and stable conditions.

Purpose of the Study:

  • To propose and demonstrate a novel open-top, common-path, intrinsically achromatic optical diffraction tomography (ODT) system.
  • To evaluate the system's performance using both coherent and partially coherent light sources for improved tomographic imaging.

Main Methods:

  • The system operates as a total-shear interferometer utilizing a Ronchi-type amplitude diffraction grating.
  • The grating is integrated directly between the camera and tube lens, eliminating the need for additional 4f imaging systems.
  • Three-beam interferograms with achromatic second harmonic generation are produced, enabling broadband light source compatibility.

Main Results:

  • The proposed ODT system is demonstrated to be low-cost, compact, and highly immune to vibrations.
  • Measurements on a 3D-printed cell phantom show that using a partially coherent light source (superluminescent diode) significantly reduces coherent noise.
  • Partially coherent illumination, combined with non-negativity constraint regularization, leads to higher fidelity 3D tomographic reconstructions.

Conclusions:

  • The developed intrinsically achromatic ODT system offers a simplified and robust approach to 3D imaging.
  • The intrinsic achromaticity allows for natural employment of broadband light sources without complex compensation.
  • Reduced coherence in illumination is advantageous for minimizing noise and enhancing the quality of tomographic reconstructions.