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Updated: Oct 22, 2025

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Published on: March 15, 2021
Common-path intrinsically achromatic optical diffraction tomography
Piotr Zdańkowski1,2,3, Julianna Winnik1,2,4, Krzysztof Patorski1
1Warsaw University of Technology, Institute of Micromechanics and Photonics, 8 Św. A. Boboli st., 02-525 Warsaw, Poland.
Abstract:
In this work we propose an open-top like common-path intrinsically achromatic optical diffraction tomography system. It operates as a total-shear interferometer and employs Ronchi-type amplitude diffraction grating, positioned in between the camera and the tube lens without an additional 4f system, generating three-beam interferograms with achromatic second harmonic. Such configuration makes the proposed system low cost, compact and immune to vibrations. We present the results of the measurements of 3D-printed cell phantom using laser diode (coherent) and superluminescent diode (partially coherent) light sources. Broadband light sources can be naturally employed without the need for any cumbersome compensation because of the intrinsic achromaticity of the interferometric recording (holograms generated by -1st and +1st conjugated diffraction orders are not affected by the illumination wavelength). The results show that the decreased coherence offers much reduced coherent noise and higher fidelity tomographic reconstruction especially when applied nonnegativity constraint regularization procedure.
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