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Printed Nanochain-Based Colorimetric Assay for Quantitative Virus Detection.
Zeying Zhang1,2, Huadong Wang1,2, Meng Su1,2
1Key Laboratory of Green Printing, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
Angewandte Chemie (International Ed. in English)
|September 8, 2021
Summary
This study presents a novel nanochain assay for rapid, label-free virus detection in biofluids. The colorimetric method offers ultrasensitive and specific identification of viral pathogens and subtypes within minutes.
Area of Science:
- Biotechnology
- Nanotechnology
- Optical Sensors
Background:
- Rapid and ultrasensitive pathogen detection is crucial for viral infection control.
- Existing methods often lack speed, sensitivity, or portability for widespread clinical use.
Purpose of the Study:
- To develop a label-free, colorimetric assay for rapid and ultrasensitive virus detection.
- To demonstrate the utility of antibody-modified nanochains for pathogen identification.
Main Methods:
- Utilized a printed nanochain assay with antibody modification for virus capture.
- Employed a label-free optical detection approach based on colorimetric changes.
- Optimized nanochain optical response for enhanced sensitivity.
Main Results:
- Achieved a detection limit of 1 PFU/μL for target viruses.
- Demonstrated rapid detection (15 min) directly from biofluids.
- Enabled real-time quantification across a wide concentration range (0-1.0×10⁵ PFU/mL).
- Showcased the ability to differentiate viral subtypes.
Conclusions:
- The nanochain assay provides a low-cost, portable, and highly sensitive platform for virus detection.
- This technology has potential for rapid clinical diagnostics and broader biomarker detection.
