Analytical Methods for Detection of Gasotransmitter Hydrogen Sulfide Released from Live Cells

Hydrogen sulfide (H₂S) plays an important role in mammals as a signaling molecule. Recently, abnormal H₂S concentration has been associated with several pathophysiological states, such as diabetes mellitus, hypertension, Alzheimer's disease, and Parkinson's disease. As regulating H₂S concentration can be a very prominent way of developing new drugs, many researchers have paid great attention to H₂S research. To understand the role of H₂S in pathophysiology and develop H₂S-based therapies, it is necessary to measure the exact concentration of H₂S within biological systems. But, H₂S is volatile and can be easily oxidized. Besides, the active sites for several biological effects of H₂S are inside the cell. Therefore, there is a need for the development of new methods for the accurate and reliable detection of H₂S within live cells. This review provides a summary of recent developments in H₂S detection methods for live cell analysis.