Related Experiment Video
Updated: Jun 27, 2026
07:27
Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
Published on: May 13, 2012
17.0K
PhaseRMiC: phase real-time microscope camera for live cell imaging
Chao Chen1, Yu-Nan Lu2, Huachuan Huang3
1Computational Optics Laboratory, School of Sciences, Jiangnan University, Wuxi, Jiangsu 214122, China.
Biomedical Optics Express
|September 13, 2021
Summary
We developed a new, affordable Phase Real-Time Microscope Camera (PhaseRMiC) for live cell imaging. This compact device offers real-time phase imaging with high accuracy, stability, and a wide field of view.
Area of Science:
- Biomedical Imaging
- Microscopy Technology
Background:
- Live cell imaging requires advanced microscopy techniques.
- Phase contrast microscopy is crucial for visualizing unstained biological samples.
Purpose of the Study:
- To design and evaluate a novel, cost-effective phase real-time microscope camera (PhaseRMiC).
- To enable high-quality, real-time phase imaging for live cell applications.
Main Methods:
- Developed a compact camera using a beam splitter, dual-CMOS board-level camera, and 3-D printed components.
- Integrated the PhaseRMiC directly into a microscope's side port.
- Tested the system for real-time phase imaging performance.
Main Results:
- Achieved a compact device size (136×91×60 mm³), comparable to commercial cameras.
- Demonstrated satisfied accuracy, good stability, and a large field of view for real-time phase imaging.
- The system proved effective for live cell imaging.
Conclusions:
- The PhaseRMiC offers a simple, cost-effective, and compact solution for live cell phase imaging.
- Its real-time imaging capabilities make it a preferred choice for biological research.
- The design facilitates easy integration with existing microscopy setups.
