Reactive Oxygen Species and the Stress Response in Octocorals

AbstractReactive oxygen species (ROS) may damage cellular components but may also contribute to signaling that mitigates damage. In this context, the role of ROS in the stress response that leads to coral bleaching was investigated in three series of experiments with octocorals Sarcothelia sp. and Sympodium sp. Using video and fluorescent microscopy, the first experiments examined ROS and symbiont migration. Colonies mildly stressed with increased temperature and light showed increases in both ROS and numbers of migrating symbionts compared with stress-free controls. Symbionts migrating in the gastrovascular lumen may escape programmed cell death and provide a reservoir of healthy symbionts once conditions return to normal. In the second series of experiments, colonies were mildly stressed with elevated temperature and light. During stress, treated colonies were incubated in seawater enriched with two concentrations of bicarbonate (1 and 3 mmol/L), while controls were incubated in normal seawater. Bicarbonate enrichment provides additional carbon for photosynthesis and at some concentrations diminished the ROS emissions of stressed colonies of Sympodium sp. and Sarcothelia sp. In all experiments, the latter species tended to exhibit more ROS. Sympodium sp. contains Cladocopium sp. symbionts, which are less tolerant of stress, while Sarcothelia sp. contains the more resistant Durusdinium sp. Indeed, in direct comparisons, Sarcothelia sp. experienced higher levels of ROS under stress-free conditions and thus is conditioned to endure the stress associated with bleaching. Generally, ROS levels provide important insight into the cnidarian stress response and should be measured more often in studies of this response.