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Proteomics

Proteomics

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Related Experiment Video

Updated: Oct 17, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Biological Applications for LC-MS-Based Proteomics.

Bradley J Smith¹, Daniel Martins-de-Souza^2,3,4,5

¹Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.

Advances in Experimental Medicine and Biology

|October 10, 2021

Summary

This summary is machine-generated.

Liquid chromatography-mass spectrometry (LC-MS) has advanced significantly, becoming more accessible and powerful for proteomics. This technology is increasingly vital in biological sciences and personalized medicine.

Keywords:

LC-MS-based proteomics Liquid chromatography-mass spectrometry Personalized medicine Proteome Proteomics

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Area of Science:

Proteomics
Mass Spectrometry
Biological Sciences

Background:

Liquid chromatography-mass spectrometry (LC-MS) has undergone continuous improvements in capabilities, sensitivity, and resolution.
Decreased costs and increased processing power have made LC-MS more accessible for various research and clinical applications.
Proteomics using LC-MS is now a popular technique in biological sciences, used in both targeted and untargeted assays.

Purpose of the Study:

To cover various applications of LC-MS-based proteomics.
To outline how these LC-MS-based proteomics applications can be executed.
To elaborate on the role of mass spectrometry in personalized medicine.

Main Methods:

Liquid chromatography-mass spectrometry (LC-MS) instrumentation.
Proteomic analysis techniques.
Mass spectrometry methods for personalized medicine.

Main Results:

LC-MS technology has become more affordable and widely available.
Increased processing power enables faster and more standardized data analysis.
Proteomics via LC-MS is integral to diverse biological research and clinical settings.

Conclusions:

LC-MS-based proteomics is a versatile tool with broad applications in pure and applied sciences, laboratories, and clinics.
The integration of LC-MS in personalized medicine is growing.
Continued evolution of mass spectrometry will further expand its presence and impact in various scientific fields.