[Study on nasal nitric oxide concentrations of 50 healthy individuals in Dalian]

Objective:To measure the normal value of nasal nitric oxide（nNO） concentration of 50 healthy adults in Dalian. By investigating the changing trend of nitric oxide accumulation concentration in the nasal cavity when changing the duration of nasal non-ventilation under quiet state, to provide ideas for the noninvasive detection of the concentration of nitric oxide in the sinus and the patency of the sinus ostium. Methods:①The left and right nNO concentrations（LnNO, RnNO） of 50 healthy subjects in Dalian were measured by Sunvou-CA2122（Wuxi）, and the LnNO₁₀, LnNO₂₀, and LnNO₃₀ were measured through the left nasal cavity when the the time of nasal non ventilation was set for 10 s, 20 s, 30 s respectively. Ten subjects with good cooperation extended the time of nasal non ventilation to 50 s, and the measured nNO value was denoted as LnNO₅₀. ②Statistical analysis was performed by SPSS 19.0, and differences between two sides of nNO were analyzed by t-test, and the trend of changes in concentration of NO accumulation in the nasal cavity when changing the duration of nasal nonventilation was investigated by One-way repeated measures ANOVA. Results:①The mean LnNO concentration in 50 healthy subjects was （383.32±126.35） ×10⁻⁹, the mean RnNO concentration was （395.26±124.32） ×10⁻⁹, and there was no significant difference between the two side（P>0.05）, and the mean nNO was （389.29±124.85） ×10⁻⁹. ②The mean nNO concentration measured at 10, 20, and 30 seconds nasal nonventilation were （984.54±477.69） ×10⁻⁹ for LnNO₁₀, （1527.32±717.25） ×10⁻⁹ for LnNO₂₀, （2183.26±946.21） ×10⁻⁹ for LnNO₃₀, and（3083.00±1905.62） ×10⁻⁹ for LnNO₅₀ measured at prolonging nasal nonventilation time to 50 seconds for ten of these subjects; The difference in the presence of increased nNO concentrations measured after prolonged nasal nonventilation was statistically significant（P<0.01）. Conclusion:①The values of nNO of 50 healthy individuals in Dalian were（389.29±124.85） ×10⁻⁹. ②In healthy individuals, nNO can be measured unilaterally instead of bilaterally. ③In quiet condition, the duration of nasal nonventilation was prolonged for a certain period of time, and the nNO concentration showed a gradually increasing trend. It is expected to provide ideas for noninvasive detection of NO concentration in nasal sinuses and patency of nasal sinuses.